US Patent No: 6,117,635

Number of patents in Portfolio can not be more than 2000

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

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Abstract

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The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This 'closed-tube' format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.

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First Claim

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Patent Owner(s)

Patent OwnerAddressTotal Patents
EMD MILLIPORE CORPORATIONBILLERICA, MA422

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bhatnagar, Satish K Gaithersburg, MD 6 439
Hohman, Robert J Gaithersburg, MD 3 323
Nazarenko, Irina A Gaithersburg, MD 3 323
Winn-Deen, Emily S Potomac, MD 49 1290

Cited Art Landscape

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