Methods of reducing the levels of protoporphyrin IX in recombinant hemoglobin preparations
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United States of America Patent
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Oct 24, 2000
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app pub date -
Oct 18, 1999
filing date -
Oct 18, 1996
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Abstract
The present invention relates to recombinant hemoglobin solutions containing reduced amounts of protoporphyrin IX and to methods of producing such hemoglobin solutions. The methods are accomplished by overexpressing the hemH gene in a suitable host cell or by increasing the production of hemH in a host cell, for example, by integration of the hemH gene into the chromosome of the host cell or by inserting multiple copies of the gene in host cells naturally having a hemH gene.
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