Method for transferring gene
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United States of America Patent
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Nov 7, 2000
Grant Date -
N/A
app pub date -
Nov 24, 1998
filing date -
Mar 26, 1997
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method for transferring a long-chain DNA into protoplasts, comprising the steps of: (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA to give a final concentration of the DNA of at least 50 .mu.g/ml; (b) collecting, washing, and culturing the protoplasts in a medium; and (c) selecting transformed cells, is provided. By this gene transfer method, long-chain DNA can be conveniently transferred into a wide variety of plant cells.
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