Interdigitated electrode arrays for liposome-enhanced immunoassay and test device
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United States of America Patent
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-
Dec 12, 2000
Grant Date -
N/A
app pub date -
Jul 15, 1999
filing date -
Sep 27, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A test device for detecting or quantifying determining an analyte in a test solution includes an absorbent material having contact, liposome lysing and electrochemical measurement portions. The contact portion is positioned for contact with and uptake of the test solution. The liposome lysing portion is segregated from the contact portion and has a liposome lysing agent bound thereto. The liposome lysing portion is further either positioned between the contact portion and the electrochemical measurement portion, or partially or completely coincides with the electrochemical measurement portion. The electrochemical measurement portion comprises a first conductor comprising a plurality of fingers disposed on the absorbent material, and a second conductor similarly comprising a plurality of fingers disposed on the absorbent material, where the fingers of the first and second conductors are interdigitated to form an array. The capture portion has a capture probe designed to at least partially hybridize with a portion of the target nucleic acid sequence bound thereto.
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