Fluorescence polarization immunoassay diagnostic method
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United States of America Patent
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Dec 12, 2000
Issued Date -
N/A
app pub date -
Oct 24, 1997
filing date -
Dec 22, 1995
priority date (Note) -
Expired
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Abstract
A method for fluorescence polarization immunoassay (FPIA) involves adding to a fluid container one or more reagents and a sample whose analyte is to be detected and measured, and then taking a first polarization measurement of the sample. More reagent is then added to the sample in the container, with no further sample addition to the container. A second or final polarization measurement of the sample is then taken, and the concentration of the analyte in the sample is then calculated based on the value of the two polarization measurements.
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