Hepes based basal nutrient medium for the isolation and culturing of stem cells
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United States of America Patent
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Dec 19, 2000
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app pub date -
Mar 18, 1999
filing date -
Jan 29, 1990
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Abstract
Novel media and methods are disclosed for the growth of stem cells. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are also useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
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