Detection and quantitation of endotoxin by fluorescence polarization
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United States of America Patent
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Jan 9, 2001
Grant Date -
N/A
app pub date -
Nov 10, 1997
filing date -
Nov 13, 1996
priority date (Note) -
Expired
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Abstract
Fluorescently labeled ENP as well as methods of preparation are disclosed. Also disclosed is a method of detecting endotoxin in a liquid sample suspected of containing endotoxin, comprising admixing said liquid sample with fluorescently labeled ENP for a time sufficient to form a complex between the labeled ENP and the endotoxin, and performing fluorescence polarization experiment on the sample, wherein a change in the fluorescence polarization indicates the presence of endotoxin.
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