Monitoring amplification of DNA during PCR
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United States of America Patent
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Jan 16, 2001
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app pub date -
Jun 4, 1997
filing date -
Jun 4, 1996
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Abstract
Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.
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