Method for the determination of homocysteine
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United States of America Patent
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Jan 16, 2001
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N/A
app pub date -
Nov 12, 1999
filing date -
Nov 12, 1999
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Abstract
The invention relates to a method for determining homocysteine concentration in samples in which homocysteine is condensed using an enzyme cystathionine .beta. sythase to form cystathionine. Pyruvate and/or ammonia are released from cystathionine by action of an enzyme, cystathionine .beta. lyase, and homocysteine is regenerated. The release of pyruvate and/or ammonia can be correlated to the concentration of homocysteine present in the sample.
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