Method for detection of target nucleic acid by analysis of stool

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United States of America Patent

PATENT NO 6177251
SERIAL NO

09328114

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Abstract

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A stool lysis buffer having a high concentration of buffer, a chelating agent, and a salt has the ability to lyse mammalian cells but not bacterial cells. Lysed cells release nucleic acids into the buffer. A particulate fraction is removed which includes the unlysed bacterial cells.

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Patent Owner(s)

Patent OwnerAddress
THE JOHNS HOPKINS UNIVERSITY3400 NORTH CHARLES STREET BALTIMORE MD 21218

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kinzler, Kenneth W Baltimore, MD 296 5437
Vogelstein, Bert Baltimore, MD 338 7135

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