Nucleic acid purification and process
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United States of America Patent
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Jan 23, 2001
Grant Date -
N/A
app pub date -
Dec 30, 1999
filing date -
Apr 23, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention disclosed is particularly directed to a process for the purification of DNA, and is especially useful for the isolation of biologically active plasmid DNA. The process is initiated by binding the DNA to silicon carbide particles, either in the presence or absence of chaotropic agents. The bound DNA is subsequently eluted from the resin in low salt buffer or water. The resulting purified DNA is substantially free of proteins and is shown to be biologically active. The scope and diversity of the process is demonstrated here since this process can be used for the isolation of RNA and genomic DNA as well as the purification of PCR products and isolation of DNA from agarose gel slices.
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