Displacing volume in field of view
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United States of America Patent
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Jan 30, 2001
Grant Date -
N/A
app pub date -
Sep 3, 1999
filing date -
Sep 3, 1999
priority date (Note) -
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status (Latency Note)
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Abstract
A method and assay system for the displacement of background from the area surrounding target material allows more sensitive detection. The invention is for use with a system in which specific targets localized at a depth within a container are detected in a liquid containing background material that produces an optical background signal. A displacement liquid is introduced that has a density selected to form a layer encompassing the target material and displacing the material that produces the background signal. The layer of the displacement liquid is sufficient to displace background material from a depth exceeding the depth of field of the detection system. The target material is then optically detected at the depth containing the target material with diminished background allowing greater detection sensitivity.
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