Method for culturing cells using wave-induced agitation
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United States of America Patent
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Feb 20, 2001
Grant Date -
N/A
app pub date -
Aug 22, 1998
filing date -
Aug 12, 1997
priority date (Note) -
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Abstract
The present invention provides a novel apparatus for culturing animal, insect, microbial, or plant cells whereby an inflated plastic bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed on rocking mechanism that moves the bag to and fro thereby inducing a wave-like motion to the liquid contained therein. This motion ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear forces or foam generation. Air is passed through the bag to provide oxygen, and sweep out evolved carbon dioxide.
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