Method for the production of sialylated oligosaccharides
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United States of America Patent
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Feb 27, 2001
Grant Date -
N/A
app pub date -
Sep 3, 1998
filing date -
Sep 3, 1998
priority date (Note) -
Expired
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Abstract
Disclosed are methods for the enzymatic synthesis of .alpha.-sialylated oligosaccharide glycosides. Specifically, in the disclosed methods, .alpha.2,3-sialyltransferase is used to transfer an analogue of sialic acid, employed as its CMP-nucleotide derivative, to the non-reducing sugar terminus of an oligosaccharide having a fucosyl group in the penultimate saccharide unit to the non-reducing sugar terminus. The analogue of sialic acid and the oligosacchairde employed in this method are selected to be compatible with the sialyltransferase employed.
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