
US Patent No: 6,197,587
Number of patents in Portfolio can not be more than 2000
Methods and tissue culture media for inducing somatic embryogenesis, agrobacterium-medicated transformation and efficient regeneration of cacao plants
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Mar 6, 2001
Issued date -
Jun 26, 1998
filing date -
09/105,648
serial no -
In Force
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Abstract
The present invention relates to improved methods of (i) inducing somatic embryogenesis from cacao tissue explants and (ii) regenerating cacao plants from somatic embryos. The invention further relates to cacao somatic embryos and plants obtained according to the methods of the invention. Novel tissue culture media adapted for use in the above-identified methods are also within the scope of the invention. The novel media of the invention include primary callus growth medium, secondary callus growth medium, embryo development medium, primary embryo conversion medium, secondary embryo conversion medium and plant regeneration medium.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
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| 4,204,366 Method of non-agricultural production of cotyledons | 8 | 1978 | |
| 4,301,619 Plant tissue produced by non-agricultural proliferation of cacao embryos | 5 | 1980 | |
| 4,291,498 Method for production of mature asexual cacao embryos, and product thereof | 5 | 1980 | |
| 4,545,147 Asexual embryogenesis of callus from theobroma cacao L. | 3 | 1983 | |
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| 5,304,725 Elite white spruce hybrids and method of production | 6 | 1992 | |
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| 5,312,801 Somatic embryogenesis and plant regeneration of cacao | 3 | 1991 | |
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| 5,530,182 Methods for production f hybrid rose plantlets from rose somatic embryos | 4 | 1994 | |
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| 5,324,646 Methods of regeneration of Medicago sativa and expressing foreign DNA in same | 12 | 1992 | |
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| 5,850,032 Method for production of plant biological products in precocious neomorphic embryoids | 25 | 1994 | |