Fluorescence-based high throughput sereening assays for protein kinases and phosphatases
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United States of America Patent
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Mar 20, 2001
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N/A
app pub date -
Dec 2, 1998
filing date -
Dec 5, 1997
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Expired
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Abstract
The invention relates to novel fluorescence-based assays for protein kinases and phosphatases which can be used in high throughput screening. The methods of the invention utilize a competitive immunoassay to determine the amount of substrate that is phosphorylated or dephosphorylated during the course of a kinase or phosphatase reaction to yield a product, as well as the phosphorylating or dephosphorylating activity of a kinase or phosphatase.
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