Composition and method for differentiation of basophils and eosinophils in blood
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United States of America Patent
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Apr 10, 2001
Grant Date -
N/A
app pub date -
Apr 28, 1999
filing date -
Apr 28, 1999
priority date (Note) -
Expired
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Abstract
This invention relates to lytic reagents and methods of using the lytic reagents for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagents selectively lyse red blood cells and certain leukocyte subpopulations, which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagents contain an ethoxylated long chain amine, a quaternary ammonium salt and an acid. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes by impedance and light scatter measurements, by impedance and radio frequency measurements, or by radio frequency and light scatter measurements.
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