Medium and matrix for long-term proliferation of cells

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United States of America Patent

PATENT NO 6231881
SERIAL NO

09113437

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Abstract

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A cell culture medium and hydrogel matrix for long term storage and proliferation of cells is provided. The cell culture medium and hydrogel matrix include an effective amount of polar amino acids, the polar amino acids selected from the group consisting of arginine, lysine, histidine, glutamic acid, and aspartic acid. The cell culture medium comprises about 5 to about 150 mM of polar amino acids. The hydrogel matrix comprises about 3 to about 150 mM of polar amino acids. Arginine and glutamic acid are preferably supplemented in the cell culture medium. Arginine, lysine, and glutamic acid are preferably supplemented in the hydrogel matrix. A method of maintaining viability and functioning of a transplant is also provided. The method of maintaining viability of a transplant includes encapsulating the cells in a hydrogel matrix and injecting the encapsulated cells into the host organism. The matrix of the present invention may also be used to promote vascularization in a transplant site prior to injection of cells.

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Patent Owner(s)

Patent OwnerAddress
ENCELLE INC545 HANNA BUILDING CLEVELAND OH 44115 UNITED STATES OF AMERICA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Klann, Richard Chris 239 E. Main St., Washington, NC 27889 21 905
Usala, Anton-Lewis 237 Buckingham Dr., Winterville, NC 28590 16 840

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