Detector for nucleic acid typing and methods of using the same
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United States of America Patent
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May 29, 2001
Grant Date -
N/A
app pub date -
Feb 8, 1999
filing date -
Apr 16, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention provides devices and methods for detecting or characterizing a nucleic acid analyte without requiring electrophoresis or the direct sequencing of analyte samples or analyte fragments. The device includes a panel or array of double stranded oligonucleotide probes immobilized on a solid support. each probe comprising a nucleotide sequence having a hypervariable number of tandem repeat sequences. Desirably, the specificity of the probes is varied with the location on the panel or array. One strand of each probe is preferably anchored at one terminus to a solid support and the opposite terminus of a second strand is not so anchored. The probes and/or the analyte are labeled by one or more reporter moieties, designed, for example, to allow for visual or instrument based detection of hybridization events.
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