Thermal and fluid cycling device for nucleic acid hybridization
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United States of America Patent
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May 29, 2001
Grant Date -
N/A
app pub date -
Aug 10, 1999
filing date -
Aug 10, 1998
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
An apparatus for automatically hybridizing nucleic acid samples is disclosed. The apparatus includes a fluid control module and a temperature control module for precisely controlling fluid contacting and temperature of a plurality of DNA samples. The DNA samples are typically arrayed on solid substrates (glass microscope slides), and the disclosed apparatus can process up to twelve slides at one time on a master unit; satellite units can be added to increase the number of slides. All slides can be processed in parallel, or may be addressed individually to undergo different hybridization protocols. Thermal control is typically by slide pairs, such that each slide pair undergoes the same temperature profile. Processes are carried out under software control by an embedded PC (personal computer). User input is by touchscreen, floppy disk drive, or external network control.
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