Quantitative method for detecting nucleotide concentration
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United States of America Patent
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Jun 26, 2001
Grant Date -
N/A
app pub date -
May 22, 1998
filing date -
Feb 27, 1997
priority date (Note) -
Expired
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Abstract
The invention provides a method for rapidly, economically and efficiently determining the concentration of a target nucleobase-containing sequence in a fluid medium using laser induced fluorescence of antisense probes. When hybridization complexes and unhybridized probes are separated prior to detection, the fluorescent intensity of the test medium is proportional to the concentration of the target sequence. When hybridization complexes and unhybridized probes are not separated prior to detection, the fluorescent intensity of the test medium is inversely proportional to the concentration of the target sequence. The method can be used to determine the concentration of a contaminant in a sample as a part of a system of quality control.
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