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Abstract

The invention relates to compounds of general structure (I) or salts thereof, wherein B is a nucleobase, X and Z independently are oxygen or sulphur, Y is hydrogen or hydroxy, which optionally may be protected, R.sub.1 is hydrocarbyl, which optionally is substituted with a functional group, R.sub.2 is hydrogen or hydrocarbyl, which optionally is substituted with a functional group, A is an electron withdrawing or electron donating group capable of moderating the acetal stability of compound (I), L.sub.1 and L.sub.2 are hydrocarbon linkers, which may be the same or different, L.sub.2, when present, being either (i) connected to L.sub.1 via the group A, or (ii) directly connected to L.sub.1, the group A then being connected to one of linkers L.sub.1 and L.sub.2, F is a dye label, Q is a coupling group for F, and l, m and n independently are 0 or 1, with the proviso that l is 1 when m is 1, and l is 1 and m is 1 when n is 1. The compounds of formula (I) are useful as deactivatable chain extension terminators. The invention also relates to the use of the compounds (I) in nucleic acid synthesis and nucleic acid sequencing as well as to a method of preparing compounds of Formula (I). ##STR1##

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First Claim