Hybridization histochemistry method for determining the presence and location in animal or plant tissue of RNA
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United States of America Patent
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Jul 24, 2001
Grant Date -
N/A
app pub date -
Jul 7, 1997
filing date -
Apr 5, 1984
priority date (Note) -
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Abstract
A method for determining the presence and location in animal or plant tissue of a specific polynucleotide population comprising: (a) preparing a section of the tissue to be examined; (b) contacting the tissue section under hybridization conditions with a synthetic, labelled oligonucleotide probe which is complementary to a representative portion of the target polynucleotide; (c) removing unhybridized probe material from the tissue section; and (d) detecting or identifying the locations in the tissue section where labelling or hybridization of the labelled probe has occurred. A diagnostic kit for use in performance of the above method is also disclosed.
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