Method of determining a functional linker for fusing globin subunts
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United States of America Patent
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Aug 14, 2001
Issued Date -
N/A
app pub date -
May 19, 1995
filing date -
May 10, 1989
priority date (Note) -
Expired
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Abstract
A functional linker for a polypeptide in which two alpha or beta globin-like domains are genetically fused is determined by screening a library of genetically fused polypeptides, in which the linker region is varied, for the ability to participate in the formation of hemoglobin-like protein, as measured by the protein's response to carbon monoxide. In a preferred embodiment, cells expressing the protein turn red as a result of carbon monoxide pressure.
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