Two-step hybridization and capture of a polynucleotide

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United States of America Patent

PATENT NO 6280952
SERIAL NO

09574561

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Abstract

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A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization conditions allow hybridization of the capture probe to the target polynucleotide, and the second hybridization conditions allow hybridization of the capture probe to the immobilized probe. The method may be used to detect the presence of a target polynucleotide in a sample by detecting the captured target polynucleotide or amplified target polynucleotide.

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Patent Owner(s)

Patent OwnerAddress
GEN-PROBE INCORPORATED10210 GENETIC CENTER DRIVE ATTN PATENT DEPT SAN DIEGO CA 92121
BIOLUCENT LLC250 CAMPUS DRIVE MARLBOROUGH MA 01752
CYTYC CORPORATION250 CAMPUS DRIVE MARLBOROUGH MA 01752
HOLOGIC INC250 CAMPUS DRIVE MARLBOROUGH MA 01752
SUROS SURGICAL SYSTEMS INC6100 TECHNOLOGY CENTER DRIVE NORTHWEST TECHNOLOGY CENTER INDIANAPOLIS IN 46278
THIRD WAVE TECHNOLOGIES INC250 CAMPUS DRIVE MARLBOROUGH MA 01752
CYTYC SURGICAL PRODUCTS LIMITED PARTNERSHIP250 CAMPUS DRIVE MARLBOROUGH MA 01752

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Becker, Michael M San Diego, CA 73 1662
Majlessi, Mehrdad San Diego, CA 16 425
Shaw, Jay H San Diego, CA 4 416
Weisburg, William G San Diego, CA 44 875

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