Rapid papanicolaou staining method for cervico-vaginal specimens
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United States of America Patent
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Sep 4, 2001
Grant Date -
N/A
app pub date -
Aug 8, 2000
filing date -
Aug 8, 2000
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method for staining a Pap test slide specimen for conventional Pap test and, particularly a ThinPrep Pap test specimen, in which a number of multiple hydrating, staining and dehydrating step are performed in an equal number of 10 to 15 dippings at about equal dip times of about 1 second. The total method time is 100 to 150 seconds. The specimen pre-staining fixative solution is a 10% buffered formalin solution and a 95% lower alcohol in a 1:2 volumetric ratio. The method simplifies the staining process and laboratory technician error, while providing an improved stain contrast with a clean background in the resultant conventional and ThinPrep Pap test stained specimens.
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