Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing
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United States of America Patent
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Sep 11, 2001
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N/A
app pub date -
Feb 11, 1998
filing date -
Feb 11, 1997
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Expired
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Abstract
There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested.
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