Antifungal proteins, DNA coding therefor, and hosts incorporating same
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United States of America Patent
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Sep 18, 2001
Grant Date -
N/A
app pub date -
Nov 20, 1996
filing date -
Feb 9, 1994
priority date (Note) -
Expired
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Abstract
The present invention provides an isolated protein obtainable from a plant source which has anti-Phytophthora activity and a molecular weight of about 60.+-.5 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein as represented by amino acids 1 to 540 of SEQ ID NO: 6, or the precursor of said protein, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also methods are provided for combatting fungi, especially Phytophthora infestans, using a protein or a host cell capable of producing the protein.
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