Self initiating single primer amplification of nucleic acids
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United States of America Patent
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Sep 25, 2001
Grant Date -
N/A
app pub date -
Apr 14, 1993
filing date -
Apr 14, 1993
priority date (Note) -
Expired
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Abstract
A method is disclosed for producing at least one copy of a pair of complementary single stranded polynucleotides. The method comprises forming, in the presence of nucleoside triphosphates and template dependent polynucleotide polymerase along each of the complementary single stranded polynucleotides, an extension of a polynucleotide primer. The polynucleotide primer is comprised of at least a sequence of 16 nucleotides terminating at its 3' end in a 2 to 9 nucleotide sequence (S1), which is complementary with the 3' ends of both of the complementary single stranded polynucleotides. The polynucleotide primer has at least an 8 nucleotide sequence (S2) that is 5' of S1, where S2 is 50 to 80% complementary to the nucleotide sequences contiguous with the 3' ends of the complementary single stranded polynucleotides. The extended polynucleotide primer and the single stranded polynucleotides are then dissociated.
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