RNA transformation vectors derived from a single-component RNA virus and contain an intervening sequence between the cap and the 5' end
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United States of America Patent
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Oct 9, 2001
Issued Date -
N/A
app pub date -
Feb 11, 2000
filing date -
Jan 16, 1998
priority date (Note) -
Expired
status (Latency Note)
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Abstract
This invention is directed to a plus strand RNA viral vector for transformation of a host organism with a foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing cis-acting viral replication elements, and allowed to infect the host organism. The RNA vector is modified to obtain infectivity by including an intervening sequence between the cap and the 5' end. The modified RNA is able to tolerate the exogeneous RNA segment without disrupting the replication of the modified RNA, in the absence of a trans-acting viral replication element in a single component plant virus host cell.
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