Characterizing nucleic acid
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United States of America Patent
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Nov 6, 2001
Grant Date -
N/A
app pub date -
Apr 10, 2000
filing date -
Jul 11, 1997
priority date (Note) -
Expired
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Abstract
The present invention involves a method for characterizing nucleic acid which comprises generating Sanger ladder nucleic acid fragments from a plurality of nucleic acid templates present in the same reaction zone, wherein at least one terminating base is present in the reaction zone. Prior to generating nucleic acid fragments, a labeled primer nucleotide or oligonucleotide is hybridized to each template. The label on each primer is specific to the template to which that primer hybridizes, thereby allowing for identification of the template. The method of the present invention further comprises identifying the length of each nucleic acid fragment produced, the template from which the fragment is derived and the terminating base of the fragment.
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