Method for the determination of a specific binding ligand using a vanadium bromoperoxidase as a signal-generating enzyme
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United States of America Patent
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Nov 27, 2001
Issued Date -
N/A
app pub date -
Oct 17, 1997
filing date -
Sep 1, 1995
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Expired
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Abstract
An analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive or sandwich assay format. The assays are carried out using a vanadium bromoperoxidase-labeled immunoreactant and a chemiluminescent signal-providing wash composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and a peroxide or a peroxide-generating reagent composition.
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