Synthesis, deprotection, analysis and purification of RNA and ribozymes
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United States of America Patent
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Mar 5, 2002
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Jun 17, 1998
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May 14, 1992
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Abstract
A process for deprotecting RNA alkylsilyl groups comprising, contacting the groups with a solution of anhydrous triethylamine-hydrogen fluoride (aHF-TEA) in triethylamine and N-methylpyrrolidine at between 60.degree. C.-70.degree. C. for 0.25-24 h.
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