Preparation of pools of nucleic acids based on representation in a sample
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United States of America Patent
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Mar 12, 2002
Grant Date -
N/A
app pub date -
Apr 27, 1998
filing date -
Apr 27, 1998
priority date (Note) -
Expired
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Abstract
The invention relates to methods for preparing nucleic acid pools useful in hybridization studies. Such methods allow hybridization conditions, such as time, temperature, ionic strength, etc., to be adjusted to increase the likelihood that hybridization to the nucleic acids within each pool is within the linear range of detection (i.e., detectable but not saturating). The methods rely on pooling nucleic acids derived from a sample, based on the degree of representation within the sample, i.e., nucleic acids having similar degrees of representation within in a sample are combined into a pool. The invention also provides arrays and kits produced from pooled nucleic acids, and an improved method for identifying a nucleic acid and/or its representation in a sample.
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