Automated system for chromosome microdissection and method of using same
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United States of America Patent
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Mar 19, 2002
Grant Date -
N/A
app pub date -
Dec 2, 1998
filing date -
Dec 2, 1997
priority date (Note) -
Expired
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Abstract
An automated system and method for chromosome microdissection involves securing a micro-tool, verifying a micro-tip position and configuration and execution of a predetermined cut pattern of a location identified by an equipment operator. The present method involves preparation of sample material on a slide placed upon a stage of an inverted optical microscope, location of the target sample for dissection, micro-tool calibration and microdissection itself. Micro-tool calibration involves the use of cameras, preferably mounted at an angle from each other, for seeing the micro-tool point, such cameras associated with vision software programs capable of locating the tool point tip at three-dimensional space. Microdissection is performed on an isolated section of chromosome placed within the appropriate three-dimensional stage. The present system and method provides reliability and ease of operation thus making microdissection widely available to laboratories.
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