Assays and probes with enzyme labels
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United States of America Patent
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Mar 26, 2002
Grant Date -
N/A
app pub date -
Sep 20, 1999
filing date -
Oct 29, 1996
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Abstract
Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phosphate and elevated temperature and reduced risk of nonspecific binding.
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