Fiber-coupled, angled-dual-illumination-axis confocal scanning microscopes for performing reflective and two-photon fluorescence imaging
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United States of America Patent
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Apr 9, 2002
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N/A
app pub date -
Nov 1, 2000
filing date -
Nov 1, 2000
priority date (Note) -
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Abstract
This invention provides an angled-dual-illumination-axis confocal scanning microscope comprising a fiber-coupled, angled-dual-illumination-axis confocal scanning head and a vertical scanning unit. The angled-dual-illumination-axis confocal scanning head is configured such that two illumination beams intersect optimally at an angle .theta. within an object and the scanning is achieved by pivoting the illumination beams and their corresponding observation beams using a single scanning element, thereby producing an arc-line scan. The vertical scanning unit causes the angled-dual-illumination-axis confocal scanning head to move towards or away from the object, thereby yielding a vertical cross-section scan of the object. The angled-dual-illumination-axis confocal scanning microscope have advantages of enhanced resolution, faster scanning, higher sensitivity and larger dynamic range of detection, a larger field of view and a longer working distance, and a compact and integrated construction.
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