Methods and compositions for organ decellularization
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United States of America Patent
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Apr 23, 2002
Grant Date -
N/A
app pub date -
Dec 29, 1999
filing date -
Dec 29, 1999
priority date (Note) -
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Abstract
The invention is directed to methods for producing a decellularized organ or part of an organ. A decellularized organ is produced using an isolated organ mechanically agitated to remove cellular membranes surrounding the isolated organ without destroying the interstitial structure of the organ. After the cellular membrane is removed, the isolated organ is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the organ. A washing fluid is used to remove the solubilized components, leaving behind a decellularized organ.
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