Cell-culture and polymer constructs
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United States of America Patent
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Apr 30, 2002
Issued Date -
N/A
app pub date -
Mar 24, 1999
filing date -
Apr 8, 1998
priority date (Note) -
Expired
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Abstract
Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.
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