Single primer PCR amplification of RNA
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United States of America Patent
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Apr 30, 2002
Grant Date -
N/A
app pub date -
Jul 17, 2000
filing date -
Jul 17, 2000
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention provides methods and compositions for amplifying RNA sequences by (a) hybridizing to a target RNA a first primer comprising a 3' target RNA hybridizing sequence and a first 5' defined amplifyable sequence; (b) extending the first primer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the first cDNA strand a second primer comprising a 3' random cDNA hybridizing sequence and a second 5' defined amplifyable sequence; (d) extending the second primer with a DNA polymerase to form a second cDNA strand; and (e) amplifying the second cDNA strand with a third primer comprising the first 5' defined amplifyable sequence.
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