Transferase and amylase, process for producing the enzymes, use thereof, and gene coding for the same

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United States of America Patent

PATENT NO 6391595
SERIAL NO

09298924

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked .alpha.-1,4 so as to transfer the .alpha.-1,4 lingages to a .alpha.-1,.alpha.-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same. Also provided are a novel amylase that has a principal activity of acting on a saccharide, as a substrate, composed of at least three sugar units wherein at least three sugar units on the reducing end side are glucose units and the linkage between the first and the second glucose units is .alpha.-1,.alpha.-1 while the linkage between the second and the third glucose units is .alpha.-1,4 so as to liberate .alpha.,.alpha.-trehalose by hydrolyzing the .alpha.-1,4 linkage and another activity of hydrolyzing the .alpha.-1,4 linkage within the molecular chain of the substrate and that liberates disaccharides and/or monosaccharides as the principal final products; a process for producing the amylase; a gene coding for the same; and a process for producing .alpha.,.alpha.-trehalose by using a combination of the transferase and the amylase.

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Patent Owner(s)

Patent OwnerAddress
KIRIN BEER KABUSHIKI KAISHANAKANO CENTRAL PARK SOUTH 10-2 NAKANO 4-CHOME NAKANO-KU TOKYO 1640001 ?1640001

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kato, Masaru Takasaki, JP 89 1097
Miura, Yutaka Takasaki, JP 33 501

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