Template chain reaction
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United States of America Patent
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Jun 11, 2002
Grant Date -
N/A
app pub date -
Mar 30, 2001
filing date -
Oct 20, 1998
priority date (Note) -
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Abstract
Methods for synthesizing oligonucleotide products and detecting single-stranded target nucleic acid sequences, are disclosed. The methods include amplifying a template sequence to produce a complementary strand hybridized to the template sequence, which is subsequently cleaved by at least one cleavage agent displacing the complementary strand as a single stranded sequence complementary to the template sequence.
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