Method for producing taxane-type diterpenes
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United States of America Patent
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Aug 6, 2002
Grant Date -
N/A
app pub date -
Jun 24, 1998
filing date -
Jun 25, 1997
priority date (Note) -
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Abstract
The present invention discloses a method for producing a taxane-type diterpene(s) by culturing cells of a taxane-type diterpene-producing plant, comprising one or both of the the following steps (a) and (b) to increase the ratio of those cell clusters suitable in size for diterpene production: (a) at least one operation for removing large cell clusters is conducted with a sieve and/or a filter during preculture of the cells to be supplied to main culture for the production of taxane-type diterpenes or at the time of cell transfer from one preculture to the subsequent preculture; (b) the cells are cultured under strong agitation conditions.
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