Method for detecting and/or quantifying a hapten in a homogeneous phase and device for implementation thereof
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United States of America Patent
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Aug 20, 2002
Grant Date -
N/A
app pub date -
Jun 11, 1999
filing date -
Apr 30, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention discloses a method for detecting and/or quantifying a hapten in a homogeneous phase, comprising the following steps: adding a known quantity of a hapten inhibitor complex to the solution containing the hapten to be detected and/or quantified; adding to the solution a quantity of antibodies corresponding to the quantity of the hapten/inhibitor complex; adding to the solution a type C .beta.-lactamase having an active site for two substrates in antigenic competition in the said active site, the first substrate being a reporter substrate capable of being transformed into a detectable and/or quantifiable product, preferably by visible UV radiation measurement, the second substrate being the hapten/inhibitor complex acting on the hydrolysis rate of the reporter substrate; detecting and/or quantifying the concentration of the product resulting from the transformation of the reporter substrate, the K.sub.m constant of the reporter substrate being at least a hundred times higher than the K.sub.m constant of the hapten/inhibitor complex, and the k.sub.cat constant being at least ten times higher than the K.sub.cat constant of the hapten/inhibitor complex.
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