DETECTION OF POLYMORPHISMS BY DENATURING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
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United States of America Patent
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Sep 17, 2002
Grant Date -
Oct 17, 2002
app pub date -
Feb 10, 2000
filing date -
Feb 10, 2000
priority date (Note) -
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Abstract
The present invention provides a method for detecting polymorphisms in a nucleic acid by preconditioning a sample of nucleic acids to completely denature the nucleic acids, e.g., via heating and/or chemical treatment, and performing high-performance liquid chromatography (HPLC) on the nucleic acid under denaturing conditions to identify any polymorphisms. The nucleic acids to be analyzed are completely denatured prior to application of the sample to a stationary reverse-phase support and throughout the HPLC process. Sample elution is also carried out under completely denaturing conditions, and the sample mixture is eluted with a mobile phase containing an ion-pairing reagent and an organic solvent.
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