Importance

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Abstract

The present invention provides improved real-time methods for amplifying nucleic acids from target microorganisms present in fruit juice comprising the steps of: a) passing fruit juice through a dissolvable filter having a pore size selected to retain the target microorganisms; b) extracting the retentate from the filter with an organic solvent selected to dissolve the filter while not interfering with the integrity of nucleic acids present in the cells of the microorganisms; c) subjecting the extracted to an organic wash step; d) subjecting the extracted retentate to an aqueous wash step; e) concentrating the retentate; and f) subjecting the nucleic acids to an amplification procedure.

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First Claim