Methods for determining single nucleotide variations and genotyping
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United States of America Patent
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Oct 1, 2002
Grant Date -
N/A
app pub date -
Dec 1, 2000
filing date -
Dec 2, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention provides methods and kits for determining the identity of a nucleotide at a variant site on a target nucleic acid. The methods begin with the template-dependent amplification of a target sequence under defined conditions to achieve selective incorporation of a nucleotide analog at the variant site. Amplification product is then subjected to limited degradation to create products having allele-specific sizes, which are subsequently separated on the basis of size. Finally, the number of products and their sizes is to assessed to determine the identity of the nucleotide(s) at the variant site and the genotype of the organism from which the target was obtained.
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