C-3' protected monomeric nucleotides and synthesis of oligonucleotides on solid support
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United States of America Patent
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Dec 3, 2002
Grant Date -
Nov 22, 2001
app pub date -
Jan 26, 2001
filing date -
Jan 28, 2000
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Immobilized nucleotide primers of this invention include a modified nucleotide tethered to a support substrate through a linking group. In particular, the modified nucleotide is constructed such that the C-5' end of the nucleotide is tetherable to the linking group and the protected C-3' end is available for further controlled modification, e.g., addition of other nucleotides in specific sequences to the immobilized nucleotide primer. Additionally, the linking group is of sufficient length to allow the immobilized nucleotide primer to be used to synthesize and screen arrays of oligonucleotides, e.g., enzymatic C-3' primer extension.
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