Generation of single-strand circular DNA from linear self-annealing segments
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United States of America Patent
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Dec 24, 2002
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N/A
app pub date -
Nov 28, 2000
filing date -
Dec 2, 1999
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Expired
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Abstract
The present invention provides a method for the rapid simultaneous production of a plurality of single-stranded DNA circles having a predetermined size and nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles.
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