Subtractive amplification kit useful in the diagnosis of genetic disease mutation or variation
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United States of America Patent
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Dec 24, 2002
Issued Date -
N/A
app pub date -
Mar 18, 1999
filing date -
Apr 29, 1996
priority date (Note) -
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Abstract
A kit is provided for preferentially amplifying target RNA in a sample of tester RNA relative to non-target RNA in the sample, the kit driver sequences complementary to the non-target tester RNA under conditions where the driver sequences hybridize to the non-target RNA, a nucleic acid primer capable of hybridizing to the target RNA under conditions suitable for extension of the nucleic acid primer; and a promoter template capable of hybridizing to a DNA that is complementary to the target RNA under conditions suitable for extension of the complementary DNA such that a functional double promoter is formed.
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